| # | Eastern Conference | MP | W | D | L | G | GD | Pts | Form |
|---|---|---|---|---|---|---|---|---|---|
| 1. | Tampa Bay | 26 | 15 | 7 | 4 | 45:23 | 22 | 52 | |
| 2. | Charleston | 26 | 14 | 4 | 8 | 52:31 | 21 | 46 | |
| 3. | Detroit | 25 | 11 | 8 | 6 | 29:19 | 10 | 41 | |
| 4. | Hartford Athletic | 26 | 9 | 12 | 5 | 29:26 | 3 | 39 | |
| 5. | Pittsburgh | 27 | 11 | 5 | 11 | 28:27 | 1 | 38 | |
| 6. | Louisville City | 26 | 10 | 7 | 9 | 43:42 | 1 | 37 | |
| 7. | Indy Eleven | 25 | 9 | 6 | 10 | 32:31 | 1 | 33 | |
| 8. | Rhode Island | 24 | 9 | 5 | 10 | 35:28 | 7 | 32 | |
| 9. | Miami FC | 26 | 7 | 9 | 10 | 32:43 | -11 | 30 | |
| 10. | Birmingham | 25 | 4 | 11 | 10 | 36:42 | -6 | 23 | |
| 11. | Loudoun | 25 | 4 | 11 | 10 | 31:43 | -12 | 23 | |
| 12. | Brooklyn | 25 | 4 | 6 | 15 | 25:49 | -24 | 18 | |
| 13. | Sporting Jax | 26 | 3 | 5 | 18 | 31:69 | -38 | 14 | |
| # | Western Conference | MP | W | D | L | G | GD | Pts | Form |
| 1. | El Paso | 26 | 14 | 5 | 7 | 45:32 | 13 | 47 | |
| 2. | New Mexico | 25 | 12 | 7 | 6 | 39:30 | 9 | 43 | |
| 3. | Lexington | 26 | 12 | 4 | 10 | 46:32 | 14 | 40 | |
| 4. | Sacramento Republic | 25 | 10 | 10 | 5 | 33:22 | 11 | 40 | |
| 5. | Colorado Springs | 26 | 11 | 6 | 9 | 40:37 | 3 | 39 | |
| 6. | Orange County SC | 26 | 10 | 9 | 7 | 35:40 | -5 | 39 | |
| 7. | FC Tulsa | 25 | 10 | 8 | 7 | 30:24 | 6 | 38 | |
| 8. | San Antonio | 26 | 9 | 11 | 6 | 29:28 | 1 | 38 | |
| 9. | Las Vegas Lights | 25 | 9 | 6 | 10 | 32:35 | -3 | 33 | |
| 10. | Oakland Roots | 26 | 8 | 9 | 9 | 33:35 | -2 | 33 | |
| 11. | Phoenix Rising | 26 | 8 | 9 | 9 | 35:38 | -3 | 33 | |
| 12. | Monterey Bay | 26 | 4 | 6 | 16 | 27:46 | -19 | 18 |